Medical Sciences (Anatomy, Physiology, Pharmacology)

Medical Sciences (Anatomy, Physiology, Pharmacology)
write a discussion about the following:
1- Benefit of mixing Lysozyme with cyclodextrine & poloxamer
2- Biological activity assay of Lysozyme
3- The effect of heating on the biological activity of lysozyme
4- If possible Add two studies related to subjects above
Prepare 5 mg/100ml
First prepare the stock solution
Lysozyme from chicken egg white
20 mg
C1 V1 = C2 V2
V1 = 5 x 100 / 20
Last lab
Lysozyme Cyclo
1 1
1 0.5
5 2.5
2.5 1.25
5 5
2.5 2.5
1.25 1.25

ABS1 ABS2
Poloxamer 188 1:1 0.104 0.107
1:0.5 0.098 0.100
Cyclodextrin 1:1 1.169 1.173
1:0.5 1.138 1.134
Now we take 0.2 ml of each of the 4 solution;
5 mg / 100 ml = 0.01 / V => V = 0.2 ml the amount we need to withdraw.
Enzyme 0.01 mg - in 0.2 ml
Enzyme 0.01 ml - in 2.5 ml bacterial suspension
0.2 ml  in 2.5 ml
0.1 ml/0.005 = 0.2 ml
0.1 ml
0.2 ml of buffer as blank –>
 0.01 % w/v  bacteria
1) 0.01 g of bacteria in 100 ml of phosphate pH 7.6
2) Blank with pH 3 buffer
λ = 450
each sample measured 3 times.
Blank 0.2 ml of pH 3 + 2.5 of the bacteria
Quick inversion  read the first reading  re-read the readings.
3) 2.5 ml of bacterial suspension + 0.2 ml of 1:1 poloxamer

0 min. 30 sec. 1 min. 1.5 min 2 min. 2.5 min. 3 min.
Poloxamer 1:1 0.01 0.008 0.007 0.006 0.005 0.004 0.004
Poloxamer 1:1 0.006 0.006 0.005 0.004 0.002 0.001 0.001
Poloxamer 1:1 0.004 0.002 0.002 0.002 0 -0.001
Poloxamer 1:0.5 0.003 0.003 0.002 0 -0.001 -0.002 -0.004
Poloxamer 1:0.5 0.006 0.004 0.004 0.003 0.002 0 -0.001
Poloxamer 1:0.5 0.005 0.005 0.004 0.003 0.002 0.001 0
Cyclodextrin 1:1 -0.002 -0.013 -0.25 -0.33 -0.41 -0.5 -0.58
Cyclodextrin 1:1 -0.002 -0.013 -0.02 -0.026 -0.036 -0.41 -0.046
Cyclodextrin 1:1 -0.006 -0.013 -0.019 -0.026 -0.033 -0.04 -0.049
Cyclodextrin 1:0.5 -0.006 -0.01 -0.016 -0.023 -0.03 -0.038 -0.045
Cyclodextrin 1:0.5 -0.002 0.009 -0.015 -0.023 -0.028 -0.037 -0.044
Cyclodextrin 1:0.5 -0.001 -0.007 -0.013 -0.021 -0.028 -0.036 -0.042
Control 5 mg/100ml +2.5 ml bact 0.054 0.056 0.055 0.054 0.053 0.052 0.051
0.008 0.008 0.007 0.006 0.004 0.004 0.002
0.01 0.011 0.008 0.008 0.007 0.005 0.003
Control 5 mg/100ml at 25 ֯c 0.014 0.018 0.017 0.015 0.016 0.015 0.014
0.013 0.014 0.013 0.012 0.012 0.011 0.009
0.021 0.022 0.023 0.021 0.02 0.02 0.02